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DNA EXTRACTION FROM SINGLE SPORES

Preparatory steps

Contamination can seriously jeopardize any effort to PCR a pure product from a fungal spore, especially if other activities involving fungal material of any kind take place anywhere in the same lab where DNA is to be extracted. All it takes is one hyphal fragment and voila! Since we are a fungal collection, extra precaution is needed—even to the point of “overkill”.
Pestle for 1.5 ml microcentrifuge tubes  Items stored in a presterilized plastic box

All items listed above are wrapped in tin foil and autoclaved at 121°C for 15 min. and immediately stored in a presterilized plastic box (cleaned with 70% alcohol).

Spore in pipette tip
Spore in pipette tip (above)

Spore in drop on tube wall
Spore in drop on tube wall (above)

Crushed spore, tube wall
Crushed spore, tube wall (above)


CDN PT1A Digital Timer/Clock 4 Event ProgrammableSpore extractions can be staggered at one minute intervals if one has multiple timers or a multi-programmable timer.