Analysis of Glomalin
Glomalin is a glycoprotein produced abundantly on hyphae and spores of arbuscular
mycorrhizal fungi in soil and in roots. Shortly after we Sara Wright and I collaborated
on development of a monoclonal antibody specific for
Paraglomus occultum, we attempted to produce monoclonal antibodies against
other AM fungi. With more recent species (e.g.
Rhizophagus intraradices), antibody cell lines were broader in specificity
but restricted to Glomeromycota. Using these cell lines, Sara Wright and co-workers
discovered they reacted specifically against glomalin. These antibodies then became
a tool to detect and quantify glomalin associated with fungal biomass and in a
wide range of soils.
Different fractions of glomalin can be extracted using different methods. PDF file explaining these methods. A sodium pyrophosphate extraction is the harshest and recovers the most soluble pool (easily extractable) as well as the total protein pool (total glomalin). Each of these latter pools can be extracted using different concentrations of sodium citrate and varying the length of autoclaving.