Mounting Spores on Glass Slides
The procedure to mount spores on slides described here are based on the assumption
that PVLG-based mountants are being used:
Add two small drops (0.5-0.75 cm in width) to slide, one of PVLG and the other of PVLG + Melzers reagent. Add spores, keeping water to a minimum if using pipette. Let stand for at least 5 minutes to let surface of drop dry slightly (increasing viscosity of edges, reducing flow when coverslip is added).
Using the blunt tip of a pencil, forcep, or needle, press directly on each coverslip over individual spores. When this is done under a dissecting microscope, each spore can be crushed to varying degrees (thus exposing hard-to-see thin flexible inner layers if present). Add more mountant to edge if needed to fill in. After 5 days at room temp or two days at 60oC, mountants harden.
The slide label should include as a minimum the following information: (1) genus name and specific epithet if known, (2) date, (3) code number for culture of origin. More detailed information on source of the specimen is needed if a code number is not linked to a specimen database or some other record-keeping method.
Slides are stored flat in aluminum trays in oak boxes and organized by genus and species. Data on each slide and slide location are stored in a FileMaker Pro relational database accessed on an computer workstation at an adjacent bench top.