The following data was provided by Dorothy Watson on a new accession (MD124) donated to INVAM at the beginning of October, 1996. It exemplifies the kinds of information needed on an accession to begin a thorough characterization that merges culture behavior and ecological properties.
Local reference: Glomus macrocarpum Ith-24MD (= Ithaca isolate #24, from Maryland)
Collector: Dorothy Watson
Last known address: Department of Soil, Crop and Atmospheric Sciences, Cornell University, Ithaca, NY 14853, USA
Collection date of original sample: April, 1995
Most detailed locality of sampling: Plot # 2: The location (in Maryland State Plane Meters) = 408690.535 Fasting and 151267.474 Northing.
Next most detailed locality information: Long-Term Cropping System Field Trials on a 40 acre field site located at the USDA Beltsville Agricultural Research Center in Beltsville, MD
County: Prince George’s
Continent: North America
Biome: Agricultural field in production for 60 years, the first 50 years in animal forage and pasture; from 1985 to 1992 in alfalfa, cut for hay and managed with conventional fertilizers and pesticides; from 1993 to 1994 in no till corn, managed with conventional fertilizers and pesticides.
Altitude: 115 It (34.5 meters)
Edaphic measurements: Keyport silt loam variant, an Aeric Ochraqunlt, clayey, mixed, mesic, somewhat poorly drained.
P: 200 kg/ha
OM%: 1.72g/lOOg total C
Texture: clayey siltloam
Any otherions: Total N: 0.13g/lOOg
pH: 6.7 (water extract)
First culture of fungal isolate:
Start date: May 9th, 1995
Sample date: October 2nd, 1995
Host: sorghum-sudangrass hybrid
Substrate: 1:1 (v/v) mixture of coarse sand to pasteurized field soil (pH = 6.9).
Type of culture: set up with five similar looking spores collected directly from site #2 field soil (collected in April)
Details: The pot used was an 8 inch conetainer.
Start date: January 12th, 1996 (after the pot culture material had been stored air-dry and cool for 2 months)
Sample date: August 28th. 1996
Host: Corn (Zea mays)
Substrate: 1:1 mixture of coarse sand to pasteurized field soil
Type of culture: 25 g of whole inoculum from the original culture was added in a layer about 4 inches under the soil surface, in a deepot. The remaining potting mixture was a 1:1 (v/v) mix of coarse sand and pasteurized soil (pH = 6.9).